Double-Layer Plaque Assay for Quantification of Enteroviruses
نویسندگان
چکیده
منابع مشابه
Double-layer plaque assay for quantification of enteroviruses.
We describe here a double-layer plaque assay for the quantification of enteroviruses, combining a monolayer plaque assay and a suspended-cell plaque assay. The double-layer assay provides significantly greater counts than other methods of virus quantification of both suspensions of pure culture viruses and naturally occurring viruses. The counts obtained by this method are approximately one ord...
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Takemoto and Kirschstein (1) found that the attenuated strain of type 1 poliovirus (sabin) contained three different plaque types when assayed with agar overlay medium containing dextran sulfate, and further investigated several biological characteristics of the virus strains with the largest and the smallest plaque types. In the present study, the observations of Takemoto and Kirschstein are r...
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Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Fred Morales, Joycelyn Powell, and Joseph L. Melnick. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin. J. Bacteriol. 91:1932-1935. 1966.-Plaque formation of 21 echoviruses (types 1-6, 9, 13, 15-19, 23-26, 29-32) and 8 coxsackieviruses (B1-6, A7, and A9) was enhanced by increased concentrati...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2004
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.70.5.2801-2805.2004